Rapiddecalcificationoftemporalboneswithpreservationofultrastructure
victoria j. maddena and miriam m. hensonb, *
a microscopy services laboratory, department of pathology and laboratory medicine, the university of north carolina, chapel hill, nc 27599, usa
b division of otolaryngology/head and neck surgery, department of surgery, cb #7090, taylor hall, the university of north carolina, chapel hill, nc 27599, usa
received 12 march 1997;
revised 13 june 1997;
accepted 17 june 1997.
available online 20 may 1998.
abstractdecalcification of temporal bones, especially from primates, has routinely required long periods of time and has been a major deterrent to many types of morphological studies. in this investigation, temporal bones from the monkey, macaca fuscata, were decalcified with ethylene diamine tetraacetic acid (edta) in a microwave oven. to isolate effects of microwaves on decalcification, tissue was fixed and embedded using routine methods; only decalcification was carried out in the microwave oven. the procedure is described in detail. instead of months, decalcification was complete in two working days. control procedures included decalcification at room temperature and use of a regular oven at a temperature equal to that reached in the microwave. the ultrastructure of cochlear tissue was equal to or better than that obtained with routine decalcification.
author keywords: temporal bone; inner ear; primate; microwave; decalcification; electron microscopy
*corresponding author. .: +1 (919) 966-3801; : +1 (919) 966-1856; : mmhenson@med.unc.edu
焦化行业有效推进超低排放改造的措施
天拓四方受邀参加2018西门子用户大会并分享MOM行业解决方案
救护车洗消站建设规范方案
食品机械分析
菲尼克斯 DC/DC转换器 2320092 初级开关模式
Rapiddecalcificationoftemporalboneswithpreservationofultrastructure
IL PN BK DI8 DO4 2SCRJ-PAC耦合器详细文章
怎么判断焊接效果的好坏
二手耙式干燥机
土壤热解吸法可以有效的去除土壤中的有机氯农药
电动调节型三片式球阀Q911F电动调节型不锈钢内螺纹球阀的特点
探索科技未知：微纳3D打印技术赋能软体机器人
过瘤胃胍基乙酸厂家
土壤盐度电导率测量仪2266FS的工作原理及使用
矿用双抗涂塑螺旋钢管有什么用途
月饼成型机加工效率与口味的双重保障
检测纸和纸板吸水性能的设备（可勃法）
一级承试资质设备的作用都有什么？
无管道净气型储药柜
网格化空气质量监测系统：打造精细化的空气质量管理体系
a microscopy services laboratory, department of pathology and laboratory medicine, the university of north carolina, chapel hill, nc 27599, usa
b division of otolaryngology/head and neck surgery, department of surgery, cb #7090, taylor hall, the university of north carolina, chapel hill, nc 27599, usa
received 12 march 1997;
revised 13 june 1997;
accepted 17 june 1997.
available online 20 may 1998.
abstractdecalcification of temporal bones, especially from primates, has routinely required long periods of time and has been a major deterrent to many types of morphological studies. in this investigation, temporal bones from the monkey, macaca fuscata, were decalcified with ethylene diamine tetraacetic acid (edta) in a microwave oven. to isolate effects of microwaves on decalcification, tissue was fixed and embedded using routine methods; only decalcification was carried out in the microwave oven. the procedure is described in detail. instead of months, decalcification was complete in two working days. control procedures included decalcification at room temperature and use of a regular oven at a temperature equal to that reached in the microwave. the ultrastructure of cochlear tissue was equal to or better than that obtained with routine decalcification.
author keywords: temporal bone; inner ear; primate; microwave; decalcification; electron microscopy
*corresponding author. .: +1 (919) 966-3801; : +1 (919) 966-1856; : mmhenson@med.unc.edu
焦化行业有效推进超低排放改造的措施
天拓四方受邀参加2018西门子用户大会并分享MOM行业解决方案
救护车洗消站建设规范方案
食品机械分析
菲尼克斯 DC/DC转换器 2320092 初级开关模式
Rapiddecalcificationoftemporalboneswithpreservationofultrastructure
IL PN BK DI8 DO4 2SCRJ-PAC耦合器详细文章
怎么判断焊接效果的好坏
二手耙式干燥机
土壤热解吸法可以有效的去除土壤中的有机氯农药
电动调节型三片式球阀Q911F电动调节型不锈钢内螺纹球阀的特点
探索科技未知：微纳3D打印技术赋能软体机器人
过瘤胃胍基乙酸厂家
土壤盐度电导率测量仪2266FS的工作原理及使用
矿用双抗涂塑螺旋钢管有什么用途
月饼成型机加工效率与口味的双重保障
检测纸和纸板吸水性能的设备（可勃法）
一级承试资质设备的作用都有什么？
无管道净气型储药柜
网格化空气质量监测系统：打造精细化的空气质量管理体系