efficacy of light-protective additive packaging in protecting milk freshness in a retail dairy case with led lighting at different light intensities
乳品防光添加剂包装在led照明不同光照强度下牛奶保鲜功效研究
aili wanga,?, catherine h. dadmunb, rachel m. handc, sean f. o'keefea, j'’nai b. phillipsa,
kemia a. andersa, susan e. duncana
a department of food science and technology, virginia polytechnic institute and state university (virginia tech), blacksburg 24061, united states
b department of chemistry, college of charleston, charleston 29424, united states
c department of food science and human nutrition, michigan state university, east lansing 48824, united states
a b s t r a c t
light emitting diodes (led) are rapidly developing as dominant lighting systems in dairy retail cases. bright light is typically chosen to best exhibit milk products. however, high intensity led lighting may create high potential for detrimental oxidation and destroying milk freshness. in this study, we investigated the interaction between led light intensity, exposure time, and packaging material on limiting milk oxidation and protecting milk freshness and vitamins. within 4 h of led light exposure at an intensity as low as 1068 lx, light-induced oxidation occurred on 2% milkfat milk with commercial packaging including glass and translucent high-density
polyethylene (hdpe) bottles. higher light intensity (> 4094 lx) and longer light exposure time (> 24 h) rapidly increased the oxidation rate in milk. polyethylene terephthalate (pet) packaging with lower oxygen permeability rate effectively reduced (p < 0.05) vitamin a degradation under low light intensity within 24 h. a combination of light-protective additive (tio2) and oxygen barrier material (pet) successfully reduced (p < .05) the loss of dissolved oxygen and riboflavin, and decreased the formation of final oxidation products in milk, as measured by thiobarbituric reactive substances (tbars), when exposed to high light intensity within
24 h. lower led light intensity in retail case was preferred by 50% of participants in a visual acceptance test; consumers are willing to consider pigmented packaging with limited visibility. results of this study provides guidance for dairy industry in choosing appropriate led lighting conditions and packaging to adequay display the milk products as well as minimize the degradation of milk nutrients and flavor.
发光二极管(led)作为乳制品零售业的主要照明系统正在迅速发展。通常选择明亮的光线来展示乳制品。然而,高强度的led照明可能产生有害氧化和破坏牛奶新鲜度高发生率。在本研究中,我们研究了led光强度、暴露时间和包装材料对限制牛奶氧化和保护牛奶新鲜度和维生素的作用。在led光照强度低至1068lx的4h内,2%的商业包装乳脂乳(包括玻璃和半透明高密度乳脂)发生光诱导氧化。聚乙烯(hdpe)瓶。较高的光照强度(>4094lx)和较长的光照时间(>24h)迅速增加了牛奶中的氧化率。低透氧率的聚对苯二甲酸乙二醇酯(pet)包装在24小时内有效降低了(p<0.05)*在低光强下的降解,光防护添加剂(tio2)和阻氧材料(pet)的组合成功降低了(p<0.05)d的损失。通过硫*活性物质(tbars)测定,当暴露在高光照强度的牛奶中时,溶解氧和核黄素,并减少牛奶中终氧化产物的形成。24小时。50%的参与者倾向于在零售情况下降低led光强度;消费者愿意考虑能见度有限的色素包装。本研究结果为乳品工业选择适当的led照明条件和包装,充分展示乳品,并将乳品营养成分和风味的降解降到低提供了指导。
2.6.2. electronic nose analysis
analysis was conducted immediay after dissolved oxygen analysis using conducting polymer electronic nose (cyranose® 320, sensigent, baldwin, ca) to prevent the loss of volatiles. settings (appendix) for the electronic nose (e-nose) analysis were established by a preliminary study assessing the draw and purge time of the instrument (amin, 2016). the cyranose® 320 measurement is based on change in resistance of each chemical sensor in the 32-sensor nosechip® when exposed to volatiles. milk sample (5 ml) was pipetted into a lean amber glass vials (20 ml) and sealed with rubber septum and cap, and the ratio between headspace volatiles and liquid sample was 3:1 in each vial. samples were held in water bath (65 °c) for a minimum of 10 min to allow equilibrium of volatiles to occur within the headspace. samples were collected by piercing the airtight rubber septum with a needle and using detector of e-nose to insert inside the vial to absorb the headspace volatiles. the process was repeated 5 times for each milk sample. e-nose data were processed with the software of pcnose® 10.11.0.76.
溶解氧分析后立即使用导电聚合物电子鼻(cyranose 320,sensigent,baldwin,ca)进行分析,以防止挥发物损失。电子鼻(e-nose)分析的设置(附录)是通过评估仪器抽吸和吹扫时间的初步研究确定的(amin,2016年)。cyranose 320的测量基于32传感器nosechip®暴露于挥发物时每个化学传感器的电阻变化。将牛奶样品(5ml)用移液管移入淡黄色玻璃瓶(20ml)中,用橡胶隔垫和瓶盖密封,每瓶顶空挥发物与液体样品的比例为3:1。将样品置于水浴(65°c)中至少10分钟,以便在顶空内发生挥发物平衡。用进样针头刺穿密封橡胶隔垫,用电子鼻检测器插入药瓶内,吸入顶空挥发物,采集样品。对每个牛奶样品重复该过程5次。电子鼻数据使用pcnose®10.11.0.76软件进行处理。
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