Invigentech转染试剂高效率转染DNA siRNA到免疫T细胞
一、浙江大学附属第二医院乳腺外科；浙江大学附属第二医院浙江省疗法肿瘤微环境与免疫重点实验室；浙江省人民医院，浙江省人民医院，杭州医学院附属人民医院，浙江省个体化医学肿瘤分子诊断与诊断重点实验室；绍兴大学附属医院甲状腺乳腺外科在2020-2-19联合发表标题为breast cancer-derived exosomes transmit lncrna snhg16 to induce cd73+γδ1 treg cells（乳腺癌衍生的外泌体传递lncrna snhg16诱导cd73 +γδ1 treg细胞）的文章到nature /sigtrans，文章已被接受；
二、本研究使用中使用invigentech（英克）公司invi dna rna 转染试剂转染质粒dna、sirna到vδ1 t免疫t细胞里面；
三、本文中转染质粒dna、sirna，转染细胞数量信息如下：
a.将全长2435 bp的序列克隆到pcr3.1载体中构建snhg16过表达载体;
b.sirna序列：snhg16-homo-349，5′gccucugcugcuaauuguutt-3'; snhg16-homo-867，5′-ccaaggagggacuguuuaatt-3′和snhg16-homo-2004，5′-cccaguguugacucaccaatt-3；
c.转染细胞用量：6孔板里面1 × 106 cells/well；
四、发表文章部分内容如下：
breast cancer-derived exosomes transmit lncrna snhg16to induce cd73+γδ1 treg cells
plasmid construction and sirna silencinga full-length 2435 bp sequence was cloned into the pcr3.1 vector to
construct the snhg16 overexpression vector. the sequences ofsnhg16-specifific sirnas were as follows: snhg16-homo-349, 5′gccucugcugcuaauuguutt-3′; snhg16-homo-867, 5′-ccaaggagggacuguuuaatt-3′ and snhg16-homo-2004, 5′-cccaguguugacucaccaatt-3′. the mir-16–5p mimics, inhibitor and negative
controls were purchased from genepharma (supplementarytable s3).
to manipulate the expression of mir-16–5p invδ1 t cells,vδ1t cells were sorted from peripheral blood and seeded into six-wellplates at 1 × 106 cells/well with 1 ml of medium supplementedwith 10% fbs, 10 μg/ml cd3, 10 μg/ml cd28 and 40 u/ml il-2.then,transfection reagent (invi dna rna transfection reagent,invigentech, usa) and mir-16–5p mimics/nc/inhibitor/inhibitornc (20 μm) were incubated with the cells for 48 h, after which thecells were collected for further experiments.
but not vδ2+t cells were revealed to comprise the majority oftils in all three bc molecular subtypes when gated on cd3(p< 0.01, fig. 1b, c). in addition, immunoflfluorescence of frozensections further confifirmed the dominant distribution of vδ1+t cells in bc (fig. 1d).
to further identify the function of breast cancer-infifiltrating γδ1t cells, bc cell lines (mcf-7, t-47d, mda-mb-231 and mda-mb-468) were co-cultured with freshly isolated vδ1+ t cells frombreast tumour tissue (e:t ratios: 1:1, 5:1 and 10:1). upon plottingthe cell growth curve, the data show that γδ1 t cells did not exert
invigentech（英克）invi dna rna转染试剂信息如下
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invi dna rna转染试剂
iv1216025
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iv1216050
0.50 ml
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invi dna rna转染试剂
iv1216075
0.75 ml
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invi dna rna转染试剂
iv1216100
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iv1216150
1.50 ml
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Invigentech转染试剂高效率转染DNA siRNA到免疫T细胞
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二、本研究使用中使用invigentech（英克）公司invi dna rna 转染试剂转染质粒dna、sirna到vδ1 t免疫t细胞里面；
三、本文中转染质粒dna、sirna，转染细胞数量信息如下：
a.将全长2435 bp的序列克隆到pcr3.1载体中构建snhg16过表达载体;
b.sirna序列：snhg16-homo-349，5′gccucugcugcuaauuguutt-3'; snhg16-homo-867，5′-ccaaggagggacuguuuaatt-3′和snhg16-homo-2004，5′-cccaguguugacucaccaatt-3；
c.转染细胞用量：6孔板里面1 × 106 cells/well；
四、发表文章部分内容如下：
breast cancer-derived exosomes transmit lncrna snhg16to induce cd73+γδ1 treg cells
plasmid construction and sirna silencinga full-length 2435 bp sequence was cloned into the pcr3.1 vector to
construct the snhg16 overexpression vector. the sequences ofsnhg16-specifific sirnas were as follows: snhg16-homo-349, 5′gccucugcugcuaauuguutt-3′; snhg16-homo-867, 5′-ccaaggagggacuguuuaatt-3′ and snhg16-homo-2004, 5′-cccaguguugacucaccaatt-3′. the mir-16–5p mimics, inhibitor and negative
controls were purchased from genepharma (supplementarytable s3).
to manipulate the expression of mir-16–5p invδ1 t cells,vδ1t cells were sorted from peripheral blood and seeded into six-wellplates at 1 × 106 cells/well with 1 ml of medium supplementedwith 10% fbs, 10 μg/ml cd3, 10 μg/ml cd28 and 40 u/ml il-2.then,transfection reagent (invi dna rna transfection reagent,invigentech, usa) and mir-16–5p mimics/nc/inhibitor/inhibitornc (20 μm) were incubated with the cells for 48 h, after which thecells were collected for further experiments.
but not vδ2+t cells were revealed to comprise the majority oftils in all three bc molecular subtypes when gated on cd3(p< 0.01, fig. 1b, c). in addition, immunoflfluorescence of frozensections further confifirmed the dominant distribution of vδ1+t cells in bc (fig. 1d).
to further identify the function of breast cancer-infifiltrating γδ1t cells, bc cell lines (mcf-7, t-47d, mda-mb-231 and mda-mb-468) were co-cultured with freshly isolated vδ1+ t cells frombreast tumour tissue (e:t ratios: 1:1, 5:1 and 10:1). upon plottingthe cell growth curve, the data show that γδ1 t cells did not exert
invigentech（英克）invi dna rna转染试剂信息如下
产品名称
货号
规格
报价
invi dna rna转染试剂
iv1216025
0.25 ml
询价
invi dna rna转染试剂
iv1216050
0.50 ml
询价
invi dna rna转染试剂
iv1216075
0.75 ml
询价
invi dna rna转染试剂
iv1216100
1.00 ml
询价
invi dna rna转染试剂
iv1216150
1.50 ml
询价
invi dna rna转染试剂
iv1216300
3.00 ml
询价
各种数控机床设备的应用
油液冷却控温装置使用维护注意事项
美国哈希SC200 多功能通用控制器操作使用
环境可靠性常见测试标准
Amplyus电泳仪（BlueGel）QP-1500-01
Invigentech转染试剂高效率转染DNA siRNA到免疫T细胞
白酒生产废水处理工艺介绍
怎么样选择好的液相色谱仪柱
新罗120T地磅（田东汽车磅）南平150吨吊秤）福安50T汽车衡维修
发热电缆电采暖解决南方室内潮湿问题
状病毒可气溶胶传播，如何正确认识？Ancon仪器助力气溶胶检测
EN12469和NSF49在生物安全柜性能和测试规矩中的比较
路面回弹弯沉值测定仪测定方法
阻旋式料位开关RC-20安装指南
模拟高海拔气压测试箱
内蒙古赤峰单机袋收尘器
定制微型迷你电子数显扭力扳手SGSX-2,0.2-2N.m
垃圾衍生燃料（RDF）制备工艺少不了破碎机
分享为什么食品厂不锈钢应釜是食品加工行业的理想选择
中国智能计量仪表行业发展困境及发展前景预测分析